› 3zio

X-ray diffraction
2.1Å resolution

minor-site specific NLS (A28)

Released:
DOI: 10.2210/pdb3zio/pdb
PDB entry 3zio coloured by chain, front view.
PDB entry 3zio coloured by chain, side view.
PDB entry 3zio coloured by chain, top view.
Source organisms:
Primary publication:
Distinctive conformation of minor site-specific nuclear localization signals bound to importin-α.
Chang CW, Couñago RM, Williams SJ, Bodén M, Kobe B
Traffic 14 1144-54 (2013)
PMID: 23910026

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156508 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 49.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 72-497; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
A28NLS Chains: B, C
Molecule details ›
Chains: B, C
Length: 12 amino acids
Theoretical weight: 1.31 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.94Å b: 90.18Å c: 100.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
2 more

4 mentions without citation

FlexPepDock lessons from CAPRI peptide-protein rounds and suggested new criteria for assessment of model quality and utility.
Marcu et al. (2017)
3 more