› 3zr0

X-ray diffraction
1.8Å resolution

Crystal structure of human MTH1 in complex with 8-oxo-dGMP

Released:
DOI: 10.2210/pdb3zr0/pdb
PDB entry 3zr0 coloured by chain, front view.
PDB entry 3zr0 coloured by chain, side view.
PDB entry 3zr0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human MTH1 and the 8-oxo-dGMP product complex.
Svensson LM, Jemth AS, Desroses M, Loseva O, Helleday T, Högbom M, Stenmark P
FEBS Lett 585 2617-21 (2011)
PMID: 21787772

Function and Biology Details

Reaction catalysed: 
2-hydroxy-dATP + H(2)O = 2-hydroxy-dAMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidized purine nucleoside triphosphate hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36639 (Residues: 1-156; Coverage: 100%)
Gene names: MTH1, NUDT1
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

2 bound ligands:
Ligand 8OG 2 x 8OG
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 59.5Å b: 67.24Å c: 79.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Targeting DNA repair, DNA metabolism and replication stress as anti-cancer strategies.
Puigvert et al. (2016)
4 more

12 mentions without citation

Targeting human MutT homolog 1 (MTH1) for cancer eradication: current progress and perspectives.
Yin et al. (2020)
11 more