› 3ztg

Solution NMR

Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)

Released:
DOI: 10.2210/pdb3ztg/pdb
PDB entry 3ztg coloured by chain, ensemble of 32 models, front view.
PDB entry 3ztg coloured by chain, ensemble of 32 models, side view.
PDB entry 3ztg coloured by chain, ensemble of 32 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of RING finger-like domain of retinoblastoma-binding protein-6 (RBBP6) suggests it functions as a U-box.
Kappo MA, Ab E, Hassem F, Atkinson RA, Faro A, Muleya V, Mulaudzi T, Poole JO, McKenzie JM, Chibi M, Moolman-Smook JC, Rees DJ, Pugh DJ
J Biol Chem 287 7146-58 (2012)
PMID: 22130672

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase RBBP6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 10.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7Z6E9 (Residues: 249-335; Coverage: 5%)
Gene names: My038, P2PR, PACT, RBBP6, RBQ1
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 45%
Refinement method: CYANA FOLLOWED BY HADDOCK
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

The oncogenic potential of small nuclear ribonucleoprotein polypeptide G: a comprehensive and perspective view.
Mabonga et al. (2019)
1 more

3 mentions without citation

Human BRCA1-BARD1 ubiquitin ligase activity counteracts chromatin barriers to DNA resection.
Densham et al. (2016)
2 more