› 3zvy

X-ray diffraction
1.95Å resolution

PHD finger of human UHRF1 in complex with unmodified histone H3 N- terminal tail

Released:
DOI: 10.2210/pdb3zvy/pdb
PDB entry 3zvy coloured by chain, front view.
PDB entry 3zvy coloured by chain, side view.
PDB entry 3zvy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The PHD finger of human UHRF1 reveals a new subgroup of unmethylated histone H3 tail readers.
Lallous N, Legrand P, McEwen AG, Ramón-Maiques S, Samama JP, Birck C
PLoS One 6 e27599 (2011)
PMID: 22096602

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159437 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase UHRF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96T88 (Residues: 296-367; Coverage: 9%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 amino acids
Theoretical weight: 934 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-9; Coverage: 6%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand TRS 2 x TRS
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 42.3Å b: 42.3Å c: 182.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.227
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 review citations

Transcriptional/epigenetic regulator CBP/p300 in tumorigenesis: structural and functional versatility in target recognition.
Wang et al. (2013)
6 more

2 mentions without citation

The PHD finger of human UHRF1 reveals a new subgroup of unmethylated histone H3 tail readers.
Lallous et al. (2011)
1 more