› 3zyc

X-ray diffraction
2.2Å resolution

DYNAMIN 1 GTPASE GED FUSION DIMER COMPLEXED WITH GMPPCP

Released:
DOI: 10.2210/pdb3zyc/pdb
PDB entry 3zyc coloured by chain, front view.
PDB entry 3zyc coloured by chain, side view.
PDB entry 3zyc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke.
Chappie JS, Mears JA, Fang S, Leonard M, Schmid SL, Milligan RA, Hinshaw JE, Dyda F
Cell 147 209-22 (2011)
PMID: 21962517

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dynamin-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 353 amino acids
Theoretical weight: 39.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05193 (Residues: 6-320, 726-750; Coverage: 39%)
Gene names: DNM, DNM1
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GCP 2 x GCP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 44.151Å b: 82.635Å c: 95.014Å
α: 90° β: 96.68° γ: 90°
R-values:
R R work R free
0.225 0.225 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

37 review citations

Fusion and fission: interlinked processes critical for mitochondrial health.
Chan DC. (2012)
36 more

16 mentions without citation

Molecular structure, function, and dynamics of clathrin-mediated membrane traffic.
Kirchhausen et al. (2014)
15 more