› 3zym

X-ray diffraction
2.03Å resolution

Structure of CALM (PICALM) in complex with VAMP8

Released:
DOI: 10.2210/pdb3zym/pdb
PDB entry 3zym coloured by chain, front view.
PDB entry 3zym coloured by chain, side view.
PDB entry 3zym coloured by chain, top view.
Source organisms:
Primary publication:
The molecular basis for the endocytosis of small R-SNAREs by the clathrin adaptor CALM.
Miller SE, Sahlender DA, Graham SC, Höning S, Robinson MS, Peden AA, Owen DJ
Cell 147 1118-31 (2011)
PMID: 22118466

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol-binding clathrin assembly protein; Vesicle-associated membrane protein 8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 310 amino acids
Theoretical weight: 35.13 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O55012 (Residues: 1-264, 276-276; Coverage: 41%)
  • Canonical: O70404 (Residues: 12-41; Coverage: 30%)
Gene names: Calm, Picalm, Vamp8
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand PO4 7 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 161.051Å b: 100.26Å c: 104.021Å
α: 90° β: 118.88° γ: 90°
R-values:
R R work R free
0.182 0.181 0.198
Expression system: Escherichia coli BL21

Quick links

Citations

39 review citations

Blood-Brain Barrier: From Physiology to Disease and Back.
Sweeney et al. (2019)
38 more

8 mentions without citation

Cargo recognition in clathrin-mediated endocytosis.
Traub et al. (2013)
7 more