› 3a4y

X-ray diffraction
2.5Å resolution

Crystal Structure of H61A mutant TTHA0252 from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb3a4y/pdb
PDB entry 3a4y coloured by chain, front view.
PDB entry 3a4y coloured by chain, side view.
PDB entry 3a4y coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Ishikawa H, Nakagawa N, Kuramitsu S, Yokoyama S, Masui R, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease TTHA0252 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLP1 (Residues: 1-431; Coverage: 100%)
Gene name: TTHA0252
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 56 x SO4
Ligand FLC 3 x FLC
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2
Unit cell:
a: 143.144Å b: 146.767Å c: 121.123Å
α: 90° β: 109.24° γ: 90°
R-values:
R R work R free
0.239 0.239 0.282
Expression system: Escherichia coli BL21(DE3)

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