› 3a60

X-ray diffraction
2.8Å resolution

Crystal structure of unphosphorylated p70S6K1 (Form I)

Released:
DOI: 10.2210/pdb3a60/pdb
PDB entry 3a60 coloured by chain, front view.
PDB entry 3a60 coloured by chain, side view.
PDB entry 3a60 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human p70 ribosomal S6 kinase-1 regulation by activation loop phosphorylation.
Sunami T, Byrne N, Diehl RE, Funabashi K, Hall DL, Ikuta M, Patel SB, Shipman JM, Smith RF, Takahashi I, Zugay-Murphy J, Iwasawa Y, Lumb KJ, Munshi SK, Sharma S
J Biol Chem 285 4587-94 (2010)
PMID: 19864428

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal protein S6 kinase beta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 36.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23443 (Residues: 75-399; Coverage: 62%)
Gene names: RPS6KB1, STK14A
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 2 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 78.6Å b: 62.893Å c: 86.992Å
α: 90° β: 94.32° γ: 90°
R-values:
R R work R free
0.21 0.21 0.268
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
7 more

20 mentions without citation

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Syed et al. (2016)
19 more