› 3a61

X-ray diffraction
3.43Å resolution

Crystal structure of unphosphorylated p70S6K1 (Form II)

Released:
DOI: 10.2210/pdb3a61/pdb
PDB entry 3a61 coloured by chain, front view.
PDB entry 3a61 coloured by chain, side view.
PDB entry 3a61 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human p70 ribosomal S6 kinase-1 regulation by activation loop phosphorylation.
Sunami T, Byrne N, Diehl RE, Funabashi K, Hall DL, Ikuta M, Patel SB, Shipman JM, Smith RF, Takahashi I, Zugay-Murphy J, Iwasawa Y, Lumb KJ, Munshi SK, Sharma S
J Biol Chem 285 4587-94 (2010)
PMID: 19864428

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal protein S6 kinase beta-1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23443 (Residues: 75-399; Coverage: 62%)
Gene names: RPS6KB1, STK14A
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 1 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 56.636Å b: 63.014Å c: 98.349Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.274 0.265 0.349
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
8 more

6 mentions without citation

Structural basis of human p70 ribosomal S6 kinase-1 regulation by activation loop phosphorylation.
Sunami et al. (2010)
5 more