› 3a62

X-ray diffraction
2.35Å resolution

Crystal structure of phosphorylated p70S6K1

Released:
DOI: 10.2210/pdb3a62/pdb
PDB entry 3a62 coloured by chain, front view.
PDB entry 3a62 coloured by chain, side view.
PDB entry 3a62 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human p70 ribosomal S6 kinase-1 regulation by activation loop phosphorylation.
Sunami T, Byrne N, Diehl RE, Funabashi K, Hall DL, Ikuta M, Patel SB, Shipman JM, Smith RF, Takahashi I, Zugay-Murphy J, Iwasawa Y, Lumb KJ, Munshi SK, Sharma S
J Biol Chem 285 4587-94 (2010)
PMID: 19864428

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal protein S6 kinase beta-1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 37.02 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23443 (Residues: 75-399; Coverage: 62%)
Gene names: RPS6KB1, STK14A
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand STU 1 x STU
Ligand MN 1 x MN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P41212
Unit cell:
a: 68.742Å b: 68.742Å c: 144.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.272
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
8 more

14 mentions without citation

Predicting new indications for approved drugs using a proteochemometric method.
Dakshanamurthy et al. (2012)
13 more