› 3ab0

X-ray diffraction
3.09Å resolution

Crystal structure of complex of the Bacillus anthracis major spore surface protein BclA with ScFv antibody fragment

Released:
DOI: 10.2210/pdb3ab0/pdb
PDB entry 3ab0 coloured by chain, front view.
PDB entry 3ab0 coloured by chain, side view.
PDB entry 3ab0 coloured by chain, top view.
Source organisms:
Primary publication:
Isolation, kinetic analysis, and structural characterization of an antibody targeting the Bacillus anthracis major spore surface protein BclA.
Nuttall SD, Wilkins ML, Streltsov VA, Pontes-Braz L, Dolezal O, Tran H, Liu CQ
Proteins 79 1306-17 (2011)
PMID: 21322055

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-125307 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
BclA C-terminal domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 136 amino acids
Theoretical weight: 13.52 KDa
Source organism: Bacillus anthracis str. '34F2 (NMRC)'
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WB0 (Residues: 311-445; Coverage: 30%)
Gene name: bclA
Sequence domains: BclA C-terminal domain
Structure domains: Jelly Rolls
Ig heavy chain V region 914 Chains: B, E
Molecule details ›
Chains: B, E
Length: 117 amino acids
Theoretical weight: 12.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18527 (Residues: 1-97; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 106 amino acids
Theoretical weight: 11.41 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: J3QMZ0 (Residues: 1-96; Coverage: 100%)
Gene name: Igkv4-54
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P213
Unit cell:
a: 125.448Å b: 125.448Å c: 125.448Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.265
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Anthrax vaccines: present status and future prospects.
Kaur et al. (2013)
3 other articles

4 mentions without citation

Multiple origins of viral capsid proteins from cellular ancestors.
Krupovic et al. (2017)
3 more