› 3acp

X-ray diffraction
2Å resolution

Crystal Structure of Yeast Rpn14, a Chaperone of the 19S Regulatory Particle of the Proteasome

Released:
DOI: 10.2210/pdb3acp/pdb
PDB entry 3acp coloured by chain, front view.
PDB entry 3acp coloured by chain, side view.
PDB entry 3acp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of yeast rpn14, a chaperone of the 19 S regulatory particle of the proteasome.
Kim S, Saeki Y, Fukunaga K, Suzuki A, Takagi K, Yamane T, Tanaka K, Mizushima T, Kato K
J Biol Chem 285 15159-15166 (2010)
PMID: 20236927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
26S proteasome regulatory subunit RPN14 Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 46.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53196 (Residues: 1-417; Coverage: 100%)
Gene names: RPN14, YGL004C
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P64
Unit cell:
a: 78.581Å b: 78.581Å c: 110.122Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure, Dynamics and Function of the 26S Proteasome.
Mao Y. (2021)
3 more

8 mentions without citation

Molecular architecture and assembly of the eukaryotic proteasome.
Tomko et al. (2013)
7 more