› 3af6

X-ray diffraction
2.6Å resolution

The crystal structure of an archaeal CPSF subunit, PH1404 from Pyrococcus horikoshii complexed with RNA-analog

Released:
DOI: 10.2210/pdb3af6/pdb
PDB entry 3af6 coloured by chain, front view.
PDB entry 3af6 coloured by chain, side view.
PDB entry 3af6 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit from Pyrococcus horikoshii.
Nishida Y, Ishikawa H, Baba S, Nakagawa N, Kuramitsu S, Masui R
Proteins 78 2395-8 (2010)
PMID: 20544974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129300 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription termination factor FttA Chain: A
Molecule details ›
Chain: A
Length: 651 amino acids
Theoretical weight: 74.06 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50112 (Residues: 1-651; Coverage: 100%)
Gene names: PH1404, fttA
Sequence domains:
Structure domains:
5'-R(*(SSU)P*(SSU)P*(SSU)P*(SSU)P*(SSU)P*(SSU))-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 6 nucleotides
Theoretical weight: 1.89 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand ZN 2 x ZN
1 modified residue:
Ligand SSU 12 x SSU

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P43212
Unit cell:
a: 86.438Å b: 86.438Å c: 237.501Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

KH-RNA interactions: back in the groove.
Nicastro et al. (2015)
5 more

3 mentions without citation

Structural biology of poly(A) site definition.
Yang et al. (2011)
2 more