› 3agn

X-ray diffraction
0.96Å resolution

Crystal Structure of Ustilago sphaerogena Ribonuclease U2 Complexed with adenosine 3'-monophosphate

Released:
DOI: 10.2210/pdb3agn/pdb
PDB entry 3agn coloured by chain, front view.
PDB entry 3agn coloured by chain, side view.
PDB entry 3agn coloured by chain, top view.
Source organism: Ustilago sphaerogena
Primary publication:
Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate.
Noguchi S
Acta Crystallogr D Biol Crystallogr 66 843-9 (2010)
PMID: 20606265

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing adenosine = an (RNA fragment)-3'-adenosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease U2 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.39 KDa
Source organism: Ustilago sphaerogena
UniProt:
  • Canonical: P00654 (Residues: 1-114; Coverage: 100%)
Gene name: RNU2
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand 3AM 1 x 3AM
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 37.88Å b: 39.789Å c: 35.884Å
α: 90° β: 114.99° γ: 90°
R-values:
R R work R free
0.118 0.117 0.127

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Citations

4 citation in other articles

The fungal ribonuclease-like effector protein CSEP0064/BEC1054 represses plant immunity and interferes with degradation of host ribosomal RNA.
Pennington et al. (2019)
3 more

4 mentions without citation

Defining the mRNA recognition signature of a bacterial toxin protein.
Schureck et al. (2015)
3 more