› 3aih

X-ray diffraction
2.1Å resolution

Human OS-9 MRH domain complexed with alpha3,alpha6-Man5

Released:
DOI: 10.2210/pdb3aih/pdb
PDB entry 3aih coloured by chain, front view.
PDB entry 3aih coloured by chain, side view.
PDB entry 3aih coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for oligosaccharide recognition of misfolded glycoproteins by OS-9 in ER-associated degradation.
Satoh T, Chen Y, Hu D, Hanashima S, Yamamoto K, Yamaguchi Y
Mol Cell 40 905-16 (2010)
PMID: 21172656

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Protein OS-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13438 (Residues: 108-229; Coverage: 19%)
Gene name: OS9
Sequence domains: Glucosidase II beta subunit-like protein
Structure domains: Mannose-6-phosphate receptor binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P6122
Unit cell:
a: 73.1Å b: 73.1Å c: 180.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.277
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The mammalian endoplasmic reticulum-associated degradation system.
Olzmann et al. (2013)
11 more

8 mentions without citation

Mannose 6-phosphate receptor homology (MRH) domain-containing lectins in the secretory pathway.
Castonguay et al. (2011)
7 more