› 3ajf

X-ray diffraction
2Å resolution

Structural insigths into dsRNA binding and RNA silencing suppression by NS3 protein of rice hoja blanca tenuivirus

Released:
DOI: 10.2210/pdb3ajf/pdb
PDB entry 3ajf coloured by chain, front view.
PDB entry 3ajf coloured by chain, side view.
PDB entry 3ajf coloured by chain, top view.
Source organism: Rice hoja blanca virus - cr
Primary publication:
Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus.
Yang X, Tan SH, Teh YJ, Yuan YA
RNA 17 903-11 (2011)
PMID: 21460234

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Suppressor of RNA silencing p3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 94 amino acids
Theoretical weight: 11.19 KDa
Source organism: Rice hoja blanca virus - cr
Expression system: Escherichia coli
UniProt:
  • Canonical: Q67897 (Residues: 21-114; Coverage: 46%)
Gene name: p3
Sequence domains: Tenuivirus movement protein
Structure domains: Tenuivirus movement protein

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 79.69Å b: 79.69Å c: 122.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.255
Expression system: Escherichia coli

Quick links

Citations

2 review citations

MicroRNAs in control of cardiac hypertrophy.
Da Costa Martins et al. (2012)
1 more

2 mentions without citation

Functional Diversity of Cytotoxic tRNase/Immunity Protein Complexes from Burkholderia pseudomallei.
Johnson et al. (2016)
1 more