› 3amk

X-ray diffraction
1.9Å resolution

Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L

Released:
DOI: 10.2210/pdb3amk/pdb
PDB entry 3amk coloured by chain, front view.
PDB entry 3amk coloured by chain, side view.
PDB entry 3amk coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Crystal structure of the branching enzyme I (BEI) from Oryza sativa L with implications for catalysis and substrate binding.
Noguchi J, Chaen K, Vu NT, Akasaka T, Shimada H, Nakashima T, Nishi A, Satoh H, Omori T, Kakuta Y, Kimura M
Glycobiology 21 1108-16 (2011)
PMID: 21493662

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 81.2 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01401 (Residues: 66-767; Coverage: 86%)
Gene names: LOC_Os06g51084, Os06g0726400, P0017G10.8-1, P0017G10.8-2, P0548E04.28-1, P0548E04.28-2, RBE1, SBE1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 43.894Å b: 114.854Å c: 66.819Å
α: 90° β: 93.98° γ: 90°
R-values:
R R work R free
0.183 0.181 0.221
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A review of starch-branching enzymes and their role in amylopectin biosynthesis.
Tetlow et al. (2014)
2 more

6 mentions without citation

Structural basis of glycogen branching enzyme deficiency and pharmacologic rescue by rational peptide design.
Froese et al. (2015)
5 more