› 3atf

X-ray diffraction
2.95Å resolution

Crystal Structure of the Kir3.2 Cytoplasmic Domain (Na+-free crystal soaked in 200 mM Cesium chloride)

Released:
DOI: 10.2210/pdb3atf/pdb
PDB entry 3atf coloured by chain, front view.
PDB entry 3atf coloured by chain, side view.
PDB entry 3atf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Interactions of cations with the cytoplasmic pores of inward rectifier K(+) channels in the closed state.
Inanobe A, Nakagawa A, Kurachi Y
J Biol Chem 286 41801-41811 (2011)
PMID: 21982822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G protein-activated inward rectifier potassium channel 2 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P48542 (Residues: 199-381; Coverage: 43%)
Gene names: Girk2, Kcnj6, Kcnj7, W
Sequence domains: Inward rectifier potassium channel C-terminal domain
Structure domains: G protein-activated inward rectifier potassium channel 1

Ligands and Environments

3 bound ligands:
Ligand CS 2 x CS
Ligand MG 1 x MG
Ligand EOH 2 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I422
Unit cell:
a: 81.732Å b: 81.732Å c: 172.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.223 0.281
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Membrane channels as integrators of G-protein-mediated signaling.
Inanobe et al. (2014)
7 other articles

3 mentions without citation

The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.
Altman et al. (2001)
2 more