› 3att

X-ray diffraction
2Å resolution

Crystal structure of Rv3168 with ATP

Released:
DOI: 10.2210/pdb3att/pdb
PDB entry 3att coloured by chain, front view.
PDB entry 3att coloured by chain, side view.
PDB entry 3att coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis Rv3168: a putative aminoglycoside antibiotics resistance enzyme.
Kim S, Nguyen CM, Kim EJ, Kim KJ
Proteins 79 2983-7 (2011)
PMID: 21905120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative aminoglycoside phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.32 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI99 (Residues: 22-378; Coverage: 94%)
Gene name: Rv3168
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand CA 2 x CA
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P212121
Unit cell:
a: 55.876Å b: 62.104Å c: 103.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.25
Expression system: Escherichia coli

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Citations

1 review citation

New Insights in to the Intrinsic and Acquired Drug Resistance Mechanisms in Mycobacteria.
Nasiri et al. (2017)
4 other articles

2 mentions without citation

Prospects for circumventing aminoglycoside kinase mediated antibiotic resistance.
Shi et al. (2013)
1 more