› 3auf

X-ray diffraction
2.07Å resolution

Crystal structure of glycinamide ribonucleotide transformylase 1 from Symbiobacterium toebii

Released:
DOI: 10.2210/pdb3auf/pdb
PDB entry 3auf coloured by chain, front view.
PDB entry 3auf coloured by chain, side view.
PDB entry 3auf coloured by chain, top view.
Source organism: Symbiobacterium toebii
Primary publication:
Structures and reaction mechanisms of the two related enzymes, PurN and PurU.
Sampei G, Kanagawa M, Baba S, Shimasaki T, Taka H, Mitsui S, Fujiwara S, Yanagida Y, Kusano M, Suzuki S, Terao K, Kawai H, Fukai Y, Nakagawa N, Ebihara A, Kuramitsu S, Yokoyama S, Kawai G
J Biochem 154 569-79 (2013)
PMID: 24108189

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123012 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.05 KDa
Source organism: Symbiobacterium toebii
Expression system: Escherichia coli
UniProt:
  • Canonical: E5RXD0 (Residues: 1-208; Coverage: 100%)
Gene names: PurN, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P3121
Unit cell:
a: 85.316Å b: 85.316Å c: 58.019Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.246
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The In Vitro Redundant Enzymes PurN and PurT Are Both Essential for Systemic Infection of Mice in Salmonella enterica Serovar Typhimurium.
Jelsbak et al. (2016)
2 more