› 3azu

X-ray diffraction
2.1Å resolution

X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA

Released:
DOI: 10.2210/pdb3azu/pdb
PDB entry 3azu coloured by chain, front view.
PDB entry 3azu coloured by chain, side view.
PDB entry 3azu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
X-ray crystal structure of the two site-specific mutants His35Gln and His35Leu of azurin from Pseudomonas aeruginosa.
Nar H, Messerschmidt A, Huber R, van de Kamp M, Canters GW
J Mol Biol 218 427-47 (1991)
PMID: 1901363

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.95 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 109.74Å b: 99.15Å c: 47.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.163 not available
Expression system: Not provided

Quick links

Citations

5 review citations

Direct electron transfer between copper-containing proteins and electrodes.
Shleev et al. (2005)
4 more

2 mentions without citation

Characterization of Lhr-Core DNA helicase and manganese- dependent DNA nuclease components of a bacterial gene cluster encoding nucleic acid repair enzymes.
Ejaz et al. (2018)
1 more