› 3b43

X-ray diffraction
3.3Å resolution

I-band fragment I65-I70 from titin

Released:
DOI: 10.2210/pdb3b43/pdb
PDB entry 3b43 coloured by chain, front view.
PDB entry 3b43 coloured by chain, side view.
PDB entry 3b43 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
A regular pattern of Ig super-motifs defines segmental flexibility as the elastic mechanism of the titin chain.
von Castelmur E, Marino M, Svergun DI, Kreplak L, Ucurum-Fotiadis Z, Konarev PV, Urzhumtsev A, Labeit D, Labeit S, Mayans O
Proc Natl Acad Sci U S A 105 1186-91 (2008)
PMID: 18212128

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 570 amino acids
Theoretical weight: 62.89 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWS0 (Residues: 1-570; Coverage: 100%)
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6522
Unit cell:
a: 141.43Å b: 141.43Å c: 166.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.22 0.267
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Muscle giants: molecular scaffolds in sarcomerogenesis.
Kontrogianni-Konstantopoulos et al. (2009)
14 more

22 mentions without citation

Titin-based tension in the cardiac sarcomere: molecular origin and physiological adaptations.
Anderson et al. (2012)
21 more