› 3b6d

X-ray diffraction
1.2Å resolution

Crystal Structure of Streptomyces Cholesterol Oxidase H447Q/E361Q mutant (1.2A)

Released:
DOI: 10.2210/pdb3b6d/pdb
PDB entry 3b6d coloured by chain, front view.
PDB entry 3b6d coloured by chain, side view.
PDB entry 3b6d coloured by chain, top view.
Source organism: Streptomyces sp. SA-COO
Primary publication:
Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase.
Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A
Protein Sci 16 2647-56 (2007)
PMID: 18029419

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.96 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAE 1 x FAE
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 51.3Å b: 73.14Å c: 63.14Å
α: 90° β: 105.05° γ: 90°
R-values:
R R work R free
0.133 0.132 0.172
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cholesterol oxidase: biochemistry and structural features.
Vrielink et al. (2009)
1 more

1 mention without citation

Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase.
Lyubimov et al. (2007)