› 3b6e

X-ray diffraction
1.6Å resolution

Crystal structure of human DECH-box RNA Helicase MDA5 (Melanoma differentiation-associated protein 5), DECH-domain

Released:
DOI: 10.2210/pdb3b6e/pdb
PDB entry 3b6e coloured by chain, front view.
PDB entry 3b6e coloured by chain, side view.
PDB entry 3b6e coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Karlberg T, Welin M, Arrowsmith CH, Berglund H, Busam RD, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Kallas A, Kotenyova T, Lehtio L, Moche M, Nilsson ME, Nordlund P, Nyman T, Persson C, Sagemark J, Svensson L, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Holmberg-Schiavone L, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interferon-induced helicase C domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BYX4 (Residues: 277-490; Coverage: 21%)
Gene names: IFIH1, MDA5, RH116
Sequence domains: Type III restriction enzyme, res subunit
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6522
Unit cell:
a: 72.83Å b: 72.83Å c: 182.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.204
Expression system: Escherichia coli

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