› 3b7e

X-ray diffraction
1.45Å resolution

Neuraminidase of A/Brevig Mission/1/1918 H1N1 strain in complex with zanamivir

Released:
DOI: 10.2210/pdb3b7e/pdb
PDB entry 3b7e coloured by chain, front view.
PDB entry 3b7e coloured by chain, side view.
PDB entry 3b7e coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structural characterization of the 1918 influenza virus H1N1 neuraminidase.
Xu X, Zhu X, Dwek RA, Stevens J, Wilson IA
J Virol 82 10493-501 (2008)
PMID: 18715929

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 42.25 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9IGQ6 (Residues: 83-467; Coverage: 82%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 5 x CA
Ligand ZMR 2 x ZMR
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2221
Unit cell:
a: 118.044Å b: 129.247Å c: 118.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.16
Expression system: Trichoplusia ni

Quick links

Citations

18 review citations

Advances in the development of influenza virus vaccines.
Krammer et al. (2015)
17 more

36 mentions without citation

In the shadow of hemagglutinin: a growing interest in influenza viral neuraminidase and its role as a vaccine antigen.
Wohlbold et al. (2014)
35 more