› 3b8k

Electron Microscopy
8.8Å resolution

Structure of the Truncated Human Dihydrolipoyl Acetyltransferase (E2)

Released:
DOI: 10.2210/pdb3b8k/pdb
PDB entry 3b8k coloured by chain, front view.
PDB entry 3b8k coloured by chain, side view.
PDB entry 3b8k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains.
Yu X, Hiromasa Y, Tsen H, Stoops JK, Roche TE, Zhou ZH
Structure 16 104-14 (2008)
PMID: 18184588
Related structures: EMD-1448

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10515 (Residues: 409-647; Coverage: 37%)
Gene names: DLAT, DLTA
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.8Å
Relevant EMDB volumes: EMD-1448
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The pyruvate dehydrogenase complexes: structure-based function and regulation.
Patel et al. (2014)
4 more

4 mentions without citation

Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains.
Yu et al. (2008)
3 more