› 3bbf

X-ray diffraction
1.7Å resolution

Crystal structure of the NM23-H2 transcription factor complex with GDP

Released:
DOI: 10.2210/pdb3bbf/pdb
PDB entry 3bbf coloured by chain, front view.
PDB entry 3bbf coloured by chain, side view.
PDB entry 3bbf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NM23-H2 may play an indirect role in transcriptional activation of c-myc gene expression but does not cleave the nuclease hypersensitive element III(1).
Dexheimer TS, Carey SS, Zuohe S, Gokhale VM, Hu X, Murata LB, Maes EM, Weichsel A, Sun D, Meuillet EJ, Montfort WR, Hurley LH
Mol Cancer Ther 8 1363-77 (2009)
PMID: 19435876

Function and Biology Details

Reactions catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase B Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 151 amino acids
Theoretical weight: 17.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22392 (Residues: 2-152; Coverage: 99%)
Gene names: NM23B, NME2
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

3 bound ligands:
Ligand GDP 6 x GDP
Ligand MG 6 x MG
Ligand DTT 2 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P212121
Unit cell:
a: 69.75Å b: 86.78Å c: 159.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.236
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Targeting G-quadruplexes in gene promoters: a novel anticancer strategy?
Balasubramanian et al. (2011)
14 more

6 mentions without citation

NME/NM23/NDPK and Histidine Phosphorylation.
Adam et al. (2020)
5 more