› 3bfb

X-ray diffraction
2.15Å resolution

Crystal structure of a pheromone binding protein from Apis mellifera in complex with the 9-keto-2(E)-decenoic acid

Released:
DOI: 10.2210/pdb3bfb/pdb
PDB entry 3bfb coloured by chain, front view.
PDB entry 3bfb coloured by chain, side view.
PDB entry 3bfb coloured by chain, top view.
Source organism: Apis mellifera
Primary publication:
Structural basis of the honey bee PBP pheromone and pH-induced conformational change.
Pesenti ME, Spinelli S, Bezirard V, Briand L, Pernollet JC, Tegoni M, Cambillau C
J Mol Biol 380 158-69 (2008)
PMID: 18508083

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pheromone-binding protein ASP1 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.19 KDa
Source organism: Apis mellifera
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9U9J6 (Residues: 26-144; Coverage: 100%)
Sequence domains: PBP/GOBP family
Structure domains: Pheromone/general odorant binding protein domain

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand 9OD 1 x 9OD
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 78.099Å b: 84.281Å c: 47.602Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.252
Expression system: Komagataella pastoris

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Citations

7 review citations

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects.
Pelosi et al. (2018)
6 more