› 3bft

X-ray diffraction
2.27Å resolution

Structure of the ligand-binding core of GluR2 in complex with the agonist (S)-TDPA at 2.25 A resolution

Released:
DOI: 10.2210/pdb3bft/pdb
PDB entry 3bft coloured by chain, front view.
PDB entry 3bft coloured by chain, side view.
PDB entry 3bft coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structures of the ligand-binding core of iGluR2 in complex with the agonists (R)- and (S)-2-amino-3-(4-hydroxy-1,2,5-thiadiazol-3-yl)propionic acid explain their unusual equipotency.
Beich-Frandsen M, Pickering DS, Mirza O, Johansen TN, Greenwood J, Vestergaard B, Schousboe A, Gajhede M, Liljefors T, Kastrup JS
J Med Chem 51 1459-63 (2008)
PMID: 18269227

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 263 amino acids
Theoretical weight: 29.22 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

5 bound ligands:
Ligand S2P 3 x S2P
Ligand ZN 5 x ZN
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 113.82Å b: 163.333Å c: 47.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.25
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Lessons from more than 80 structures of the GluA2 ligand-binding domain in complex with agonists, antagonists and allosteric modulators.
Pøhlsgaard et al. (2011)
1 other article

1 mention without citation

Size matters in activation/inhibition of ligand-gated ion channels.
Du et al. (2012)