› 3bg9

X-ray diffraction
3Å resolution

Crystal Structure of Human Pyruvate Carboxylase (missing the biotin carboxylase domain at the N-terminus) F1077A Mutant

Released:
DOI: 10.2210/pdb3bg9/pdb
PDB entry 3bg9 coloured by chain, front view.
PDB entry 3bg9 coloured by chain, side view.
PDB entry 3bg9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction.
Xiang S, Tong L
Nat Struct Mol Biol 15 295-302 (2008)
PMID: 18297087

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate carboxylase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 718 amino acids
Theoretical weight: 78.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11498 (Residues: 482-1178; Coverage: 59%)
Gene name: PC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 107.54Å b: 107.54Å c: 524.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.189 0.236
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Regulation of pyruvate metabolism and human disease.
Gray et al. (2014)
10 more

3 mentions without citation

Myasthenia Gravis: Pathogenic Effects of Autoantibodies on Neuromuscular Architecture.
Koneczny et al. (2019)
2 more