› 3bjh

X-ray diffraction
1.6Å resolution

Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.

Released:
DOI: 10.2210/pdb3bjh/pdb
PDB entry 3bjh coloured by chain, front view.
PDB entry 3bjh coloured by chain, side view.
PDB entry 3bjh coloured by chain, top view.
Source organism: Apis mellifera
Primary publication:
Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L.
Lartigue A, Gruez A, Briand L, Blon F, Bézirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C
J Biol Chem 279 4459-64 (2004)
PMID: 14594955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pheromone-binding protein ASP1 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.19 KDa
Source organism: Apis mellifera
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9U9J6 (Residues: 26-144; Coverage: 100%)
Sequence domains: PBP/GOBP family
Structure domains: Pheromone/general odorant binding protein domain

Ligands and Environments

2 bound ligands:
Ligand NBB 1 x NBB
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 75.386Å b: 86.289Å c: 50.698Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.171 0.206
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects.
Pelosi et al. (2018)
4 more

3 mentions without citation

Crystallographic observation of pH-induced conformational changes in the Amyelois transitella pheromone-binding protein AtraPBP1.
di Luccio et al. (2013)
2 more