› 3bmb

X-ray diffraction
1.91Å resolution

Crystal structure of a new RNA polymerase interacting protein

Released:
DOI: 10.2210/pdb3bmb/pdb
PDB entry 3bmb coloured by chain, front view.
PDB entry 3bmb coloured by chain, side view.
PDB entry 3bmb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli Rnk, a new RNA polymerase-interacting protein.
Lamour V, Rutherford ST, Kuznedelov K, Ramagopal UA, Gourse RL, Severinov K, Darst SA
J Mol Biol 383 367-79 (2008)
PMID: 18760284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nucleoside diphosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 14.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFW4 (Residues: 1-136; Coverage: 100%)
Gene names: JW0602, b0610, rnk
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 10 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: C2221
Unit cell:
a: 68.5Å b: 133.4Å c: 66.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.237
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial Transcription as a Target for Antibacterial Drug Development.
Ma et al. (2016)
4 more

3 mentions without citation

Crystal structure of Escherichia coli Rnk, a new RNA polymerase-interacting protein.
Lamour et al. (2008)
2 more