› 3bni

X-ray diffraction
2.3Å resolution

Crystal structure of TetR-family transcriptional regulator from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb3bni/pdb
PDB entry 3bni coloured by chain, front view.
PDB entry 3bni coloured by chain, side view.
PDB entry 3bni coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Entry authors: Osipiuk J, Xu X, Gu J, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 25.78 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CK21 (Residues: 1-205; Coverage: 100%)
Gene names: SCI11.01c, SCI30A.33c, SCO1712
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 46.091Å b: 66.354Å c: 125.083Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.249
Expression system: Escherichia coli BL21(DE3)

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