› 3bow

X-ray diffraction
2.4Å resolution

Structure of M-calpain in complex with Calpastatin

Released:
DOI: 10.2210/pdb3bow/pdb
PDB entry 3bow coloured by chain, front view.
PDB entry 3bow coloured by chain, side view.
PDB entry 3bow coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Calcium-bound structure of calpain and its mechanism of inhibition by calpastatin.
Hanna RA, Campbell RL, Davies PL
Nature 456 409-12 (2008)
PMID: 19020623

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150972 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Calpain-2 catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 714 amino acids
Theoretical weight: 81.58 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07009 (Residues: 1-700; Coverage: 100%)
Gene name: Capn2
Sequence domains:
Structure domains:
Calpain small subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 184 amino acids
Theoretical weight: 21.3 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64537 (Residues: 88-270; Coverage: 68%)
Gene names: Capn4, Capns1, Css1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Calpastatin Chain: C
Molecule details ›
Chain: C
Length: 95 amino acids
Theoretical weight: 10.77 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P27321 (Residues: 571-664; Coverage: 13%)
Gene name: Cast
Sequence domains: Calpain inhibitor

Ligands and Environments

1 bound ligand:
Ligand CA 10 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 67.477Å b: 66.985Å c: 108.656Å
α: 90° β: 100.76° γ: 90°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Botulinum neurotoxin: a marvel of protein design.
Montal M. (2010)
43 more

19 mentions without citation

Regulation and physiological roles of the calpain system in muscular disorders.
Sorimachi et al. (2012)
18 more