› 3btu

X-ray diffraction
2.85Å resolution

Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S2) [E351K]

Released:
DOI: 10.2210/pdb3btu/pdb
PDB entry 3btu coloured by chain, front view.
PDB entry 3btu coloured by chain, side view.
PDB entry 3btu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
NADP regulates the yeast GAL induction system.
Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L
Science 319 1090-2 (2008)
PMID: 18292341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose/lactose metabolism regulatory protein GAL80 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 438 amino acids
Theoretical weight: 48.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P04387 (Residues: 1-435; Coverage: 100%)
Gene names: GAL80, YM9827.01, YM9958.12, YML051W
Sequence domains: Oxidoreductase family, NAD-binding Rossmann fold
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 495.32Å b: 84.862Å c: 66.46Å
α: 90° β: 98.9° γ: 90°
R-values:
R R work R free
0.228 0.228 0.278
Expression system: Escherichia coli

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Citations

7 review citations

Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae.
Conrad et al. (2014)
6 more