› 3bus

X-ray diffraction
2.65Å resolution

Crystal Structure of RebM

Released:
DOI: 10.2210/pdb3bus/pdb
PDB entry 3bus coloured by chain, front view.
PDB entry 3bus coloured by chain, side view.
PDB entry 3bus coloured by chain, top view.
Source organism: Lentzea aerocolonigenes
Primary publication:
Structure and mechanism of the rebeccamycin sugar 4'-O-methyltransferase RebM.
Singh S, McCoy JG, Zhang C, Bingman CA, Phillips GN, Thorson JS
J Biol Chem 283 22628-36 (2008)
PMID: 18502766

Function and Biology Details

Reaction catalysed: 
4'-demethylrebeccamycin + S-adenosyl-L-methionine = rebeccamycin + S-adenosyl-L-homocysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Demethylrebeccamycin-D-glucose O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 29.79 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KZ94 (Residues: 11-283; Coverage: 97%)
Gene name: rebM
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 119.153Å b: 119.153Å c: 84.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.26
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
2 more

14 mentions without citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
13 more