› 3bx9

X-ray diffraction
1.8Å resolution

Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 2.0

Released:
DOI: 10.2210/pdb3bx9/pdb
PDB entry 3bx9 coloured by chain, front view.
PDB entry 3bx9 coloured by chain, side view.
PDB entry 3bx9 coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
A crystallographic study of bright far-red fluorescent protein mKate reveals pH-induced cis-trans isomerization of the chromophore.
Pletnev S, Shcherbo D, Chudakov DM, Pletneva N, Merzlyak EM, Wlodawer A, Dauter Z, Pletnev V
J Biol Chem 283 28980-7 (2008)
PMID: 18682399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-163440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Far-red fluorescent protein mKate Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.64 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CIT 6 x CIT
Ligand GOL 2 x GOL
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6522
Unit cell:
a: 67.852Å b: 67.852Å c: 413.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.247
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
9 more

4 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
3 more