› 3bxa

X-ray diffraction
1.75Å resolution

Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 4.2

Released:
DOI: 10.2210/pdb3bxa/pdb
PDB entry 3bxa coloured by chain, front view.
PDB entry 3bxa coloured by chain, side view.
PDB entry 3bxa coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
A crystallographic study of bright far-red fluorescent protein mKate reveals pH-induced cis-trans isomerization of the chromophore.
Pletnev S, Shcherbo D, Chudakov DM, Pletneva N, Merzlyak EM, Wlodawer A, Dauter Z, Pletnev V
J Biol Chem 283 28980-7 (2008)
PMID: 18682399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-163442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Far-red fluorescent protein mKate Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.64 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4
Unit cell:
a: 98.181Å b: 98.181Å c: 106.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.233
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
8 more

6 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
5 more