› 3c08

X-ray diffraction
2.15Å resolution

Crystal structure the Fab fragment of matuzumab/EMD72000 (Fab72000)

Released:
DOI: 10.2210/pdb3c08/pdb
PDB entry 3c08 coloured by chain, front view.
PDB entry 3c08 coloured by chain, side view.
PDB entry 3c08 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Matuzumab binding to EGFR prevents the conformational rearrangement required for dimerization.
Schmiedel J, Blaukat A, Li S, Knöchel T, Ferguson KM
Cancer Cell 13 365-73 (2008)
PMID: 18394559

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234856 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Matuzumab Fab Light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Matuzumab Fab Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 24.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 56.821Å b: 61.377Å c: 102.675Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.264
Expression system: Mus musculus

Quick links

Citations

28 review citations

Recombinant antibody therapeutics: the impact of glycosylation on mechanisms of action.
Jefferis R. (2009)
27 more

5 mentions without citation

Matuzumab binding to EGFR prevents the conformational rearrangement required for dimerization.
Schmiedel et al. (2008)
4 more