› 3c3w

X-ray diffraction
2.2Å resolution

Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR

Released:
DOI: 10.2210/pdb3c3w/pdb
PDB entry 3c3w coloured by chain, front view.
PDB entry 3c3w coloured by chain, side view.
PDB entry 3c3w coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structures of the response regulator DosR from Mycobacterium tuberculosis suggest a helix rearrangement mechanism for phosphorylation activation.
Wisedchaisri G, Wu M, Sherman DR, Hol WG
J Mol Biol 378 227-42 (2008)
PMID: 18353359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional activator DevR/DosR Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.4 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMF9 (Residues: 1-217; Coverage: 100%)
Gene names: Rv3133c, devR, dosR
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 48.107Å b: 98.08Å c: 53.71Å
α: 90° β: 93.81° γ: 90°
R-values:
R R work R free
0.189 0.186 0.249
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Adaptation to environmental stimuli within the host: two-component signal transduction systems of Mycobacterium tuberculosis.
Bretl et al. (2011)
2 more

13 mentions without citation

Receiver domain structure and function in response regulator proteins.
Bourret RB. (2010)
12 more