› 3c6m

X-ray diffraction
2.45Å resolution

Crystal structure of human spermine synthase in complex with spermine and 5-methylthioadenosine

Released:
DOI: 10.2210/pdb3c6m/pdb
PDB entry 3c6m coloured by chain, front view.
PDB entry 3c6m coloured by chain, side view.
PDB entry 3c6m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human spermine synthase: implications of substrate binding and catalytic mechanism.
Wu H, Min J, Zeng H, McCloskey DE, Ikeguchi Y, Loppnau P, Michael AJ, Pegg AE, Plotnikov AN
J Biol Chem 283 16135-46 (2008)
PMID: 18367445

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + spermidine = S-methyl-5'-thioadenosine + spermine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spermine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52788 (Residues: 5-366; Coverage: 99%)
Gene name: SMS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SPM 4 x SPM
Ligand MTA 4 x MTA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P32
Unit cell:
a: 88.262Å b: 88.262Å c: 197.668Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.271
Expression system: Escherichia coli

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Citations

16 review citations

Mammalian polyamine metabolism and function.
Pegg AE. (2009)
15 more

6 mentions without citation

Functions of Polyamines in Mammals.
Pegg AE. (2016)
5 more