› 3c7x

X-ray diffraction
1.7Å resolution

Hemopexin-like domain of matrix metalloproteinase 14

Released:
DOI: 10.2210/pdb3c7x/pdb
PDB entry 3c7x coloured by chain, front view.
PDB entry 3c7x coloured by chain, side view.
PDB entry 3c7x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The dimer interface of the membrane type 1 matrix metalloproteinase hemopexin domain: crystal structure and biological functions.
Tochowicz A, Goettig P, Evans R, Visse R, Shitomi Y, Palmisano R, Ito N, Richter K, Maskos K, Franke D, Svergun D, Nagase H, Bode W, Itoh Y
J Biol Chem 286 7587-600 (2011)
PMID: 21193411

Function and Biology Details

Reaction catalysed: 
Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-156156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix metalloproteinase-14 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 23.13 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P50281 (Residues: 316-511; Coverage: 35%)
Gene name: MMP14
Sequence domains: Hemopexin
Structure domains: Hemopexin-like domain

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P3121
Unit cell:
a: 77.21Å b: 77.21Å c: 66.819Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.185 0.207
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Interstitial collagen catabolism.
Fields GB. (2013)
7 more

17 mentions without citation

Inhibition of matrix metalloproteinase 14 (MMP-14)-mediated cancer cell migration.
Zarrabi et al. (2011)
16 more