› 3cbg

X-ray diffraction
2Å resolution

Functional and Structural Characterization of a Cationdependent O-Methyltransferase from the Cyanobacterium Synechocystis Sp. Strain PCC 6803

Released:
DOI: 10.2210/pdb3cbg/pdb
PDB entry 3cbg coloured by chain, front view.
PDB entry 3cbg coloured by chain, side view.
PDB entry 3cbg coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Functional and structural characterization of a cation-dependent O-methyltransferase from the cyanobacterium Synechocystis sp. strain PCC 6803.
Kopycki JG, Stubbs MT, Brandt W, Hagemann M, Porzel A, Schmidt J, Schliemann W, Zenk MH, Vogt T
J Biol Chem 283 20888-96 (2008)
PMID: 18502765

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.74 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55813 (Residues: 1-220; Coverage: 100%)
Gene name: slr0095
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand SAH 1 x SAH
Ligand FER 1 x FER
Ligand 4FE 1 x 4FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3121
Unit cell:
a: 57.622Å b: 57.622Å c: 119.834Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.152 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
1 more

4 mentions without citation

An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.
Oliva Chávez et al. (2015)
3 more