› 3cfw

X-ray diffraction
2.2Å resolution

L-selectin lectin and EGF domains

Released:
DOI: 10.2210/pdb3cfw/pdb
PDB entry 3cfw coloured by chain, front view.
PDB entry 3cfw coloured by chain, side view.
PDB entry 3cfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Glycan Bound to the Selectin Low Affinity State Engages Glu-88 to Stabilize the High Affinity State under Force.
Mehta-D'souza P, Klopocki AG, Oganesyan V, Terzyan S, Mather T, Li Z, Panicker SR, Zhu C, McEver RP
J Biol Chem 292 2510-2518 (2017)
PMID: 28011641

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
L-selectin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 19.21 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P14151 (Residues: 39-194; Coverage: 45%)
Gene names: LNHR, LYAM1, SELL
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: I23
Unit cell:
a: 118.477Å b: 118.477Å c: 118.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.248
Expression system: Cricetulus griseus

Quick links

Citations

5 review citations

A head-to-tail view of L-selectin and its impact on neutrophil behaviour.
Ivetic A. (2018)
4 more

14 mentions without citation

Rolling cell adhesion.
McEver et al. (2010)
13 more