EC 6.2.1.45: E1 ubiquitin-activating enzyme
Reaction catalysed:
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Systematic name:
Ubiquitin:[E1 ubiquitin-activating enzyme] ligase (AMP-forming)
Alternative Name(s):
- E1
- Ubiquitin activating enzyme
- Ubiquitin-activating enzyme E1
GO terms
Biochemical function:
Biological process:
Cellular component:
Sequence families
Protein families (Pfam)
![The deposited structure of PDB entry 3cmm contains 4 copies of Pfam domain PF00899 (ThiF family) in Ubiquitin-activating enzyme E1 1. Showing 2 copies in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_Pfam_PF00899_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 4 copies of Pfam domain <span class='highlight'>PF00899 (ThiF family)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 2 copies in chain <span class='highlight'>B [auth C]</span>.")
![The deposited structure of PDB entry 3cmm contains 2 copies of Pfam domain PF00240 (Ubiquitin family) in Ubiquitin. Showing 1 copy in chain C [auth B].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_2_B_Pfam_PF00240_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of Pfam domain <span class='highlight'>PF00240 (Ubiquitin family)</span> in <span class='highlight'>Ubiquitin</span>. Showing 1 copy in chain <span class='highlight'>C [auth B]</span>.")
![The deposited structure of PDB entry 3cmm contains 2 copies of Pfam domain PF09358 (Ubiquitin fold domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_Pfam_PF09358_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of Pfam domain <span class='highlight'>PF09358 (Ubiquitin fold domain)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
![The deposited structure of PDB entry 3cmm contains 2 copies of Pfam domain PF10585 (Ubiquitin-activating enzyme, SCCH domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_Pfam_PF10585_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of Pfam domain <span class='highlight'>PF10585 (Ubiquitin-activating enzyme, SCCH domain)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
![The deposited structure of PDB entry 3cmm contains 2 copies of Pfam domain PF16191 (Ubiquitin-activating enzyme E1 four-helix bundle) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_Pfam_PF16191_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of Pfam domain <span class='highlight'>PF16191 (Ubiquitin-activating enzyme E1 four-helix bundle)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
![The deposited structure of PDB entry 3cmm contains 2 copies of Pfam domain PF16190 (Ubiquitin-activating enzyme E1 FCCH domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_Pfam_PF16190_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of Pfam domain <span class='highlight'>PF16190 (Ubiquitin-activating enzyme E1 FCCH domain)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
InterPro annotations
IPR038252
Domain description:
Ubiquitin-activating enzyme E1, C-terminal domain superfamily
Occurring in:
Occurring in:
Structure domains
CATH domains
3.40.50.720
Class:
Alpha Beta
Architecture:
3-Layer(aba) Sandwich
Topology:
Rossmann fold
Homology:
NAD(P)-binding Rossmann-like Domain
Occurring in:
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 3.40.50.720 (Rossmann fold) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_CATH_3.40.50.720_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>3.40.50.720 (Rossmann fold)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
3.40.50.12550
Class:
Alpha Beta
Architecture:
3-Layer(aba) Sandwich
Topology:
Rossmann fold
Homology:
Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2
Occurring in:
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 3.40.50.12550 (Rossmann fold) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_CATH_3.40.50.12550_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>3.40.50.12550 (Rossmann fold)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
3.10.20.90
Class:
Alpha Beta
Architecture:
Roll
Topology:
Ubiquitin-like (UB roll)
Homology:
Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Occurring in:
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 3.10.20.90 (Ubiquitin-like (UB roll)) in Ubiquitin. Showing 1 copy in chain C [auth B].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_2_B_CATH_3.10.20.90_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>3.10.20.90 (Ubiquitin-like (UB roll))</span> in <span class='highlight'>Ubiquitin</span>. Showing 1 copy in chain <span class='highlight'>C [auth B]</span>.")
3.10.290.60
Class:
Alpha Beta
Architecture:
Roll
Topology:
Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A
Homology:
Ubiquitin-activating enzyme E1, UFD domain
Occurring in:
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 3.10.290.60 (Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_CATH_3.10.290.60_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>3.10.290.60 (Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
1.10.10.2660
Class:
Mainly Alpha
Architecture:
Orthogonal Bundle
Topology:
Arc Repressor Mutant, subunit A
Homology:
Ubiquitin-activating enzyme E1, SCCH domain
Occurring in:
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 1.10.10.2660 (Arc Repressor Mutant, subunit A) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_CATH_1.10.10.2660_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>1.10.10.2660 (Arc Repressor Mutant, subunit A)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
2.40.30.180
Class:
Mainly Beta
Architecture:
Beta Barrel
Topology:
Elongation Factor Tu (Ef-tu); domain 3
Homology:
Ubiquitin-activating enzyme E1, FCCH domain
Occurring in:
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 2.40.30.180 (Elongation Factor Tu (Ef-tu); domain 3) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_CATH_2.40.30.180_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>2.40.30.180 (Elongation Factor Tu (Ef-tu); domain 3)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
![The deposited structure of PDB entry 3cmm contains 2 copies of CATH domain 3.50.50.80 (FAD/NAD(P)-binding domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain B [auth C].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_1_C_CATH_3.50.50.80_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of CATH domain <span class='highlight'>3.50.50.80 (FAD/NAD(P)-binding domain)</span> in <span class='highlight'>Ubiquitin-activating enzyme E1 1</span>. Showing 1 copy in chain <span class='highlight'>B [auth C]</span>.")
SCOP annotation![The deposited structure of PDB entry 3cmm contains 2 copies of SCOP domain 54237 (Ubiquitin-related) in Ubiquitin. Showing 1 copy in chain C [auth B].](https://www.ebi.ac.uk/pdbe/static/entry/3cmm_2_B_SCOP_54237_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3cmm</span> contains 2 copies of SCOP domain <span class='highlight'>54237 (Ubiquitin-related)</span> in <span class='highlight'>Ubiquitin</span>. Showing 1 copy in chain <span class='highlight'>C [auth B]</span>.")