› 3cog

X-ray diffraction
2Å resolution

Crystal structure of human cystathionase (Cystathionine gamma lyase) in complex with DL-propargylglycine

Released:
DOI: 10.2210/pdb3cog/pdb
PDB entry 3cog coloured by chain, front view.
PDB entry 3cog coloured by chain, side view.
PDB entry 3cog coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the inhibition mechanism of human cystathionine gamma-lyase, an enzyme responsible for the production of H(2)S.
Sun Q, Collins R, Huang S, Holmberg-Schiavone L, Anand GS, Tan CH, van-den-Berg S, Deng LW, Moore PK, Karlberg T, Sivaraman J
J Biol Chem 284 3076-3085 (2009)
PMID: 19019829

Function and Biology Details

Reactions catalysed: 
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
(1a) L-homocysteine = hydrogen sulfide + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine gamma-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 403 amino acids
Theoretical weight: 44.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32929 (Residues: 1-402; Coverage: 99%)
Gene name: CTH
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 3 x PLP
4 bound ligands:
Ligand NO3 3 x NO3
Ligand 2AG 3 x 2AG
Ligand GOL 5 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 105.35Å b: 107.22Å c: 153.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.158 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Actions and interactions of nitric oxide, carbon monoxide and hydrogen sulphide in the cardiovascular system and in inflammation--a tale of three gases!
Li et al. (2009)
29 more

18 mentions without citation

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
17 more