› 3cql

X-ray diffraction
1.5Å resolution

Crystal Structure of GH family 19 chitinase from Carica papaya

Released:
DOI: 10.2210/pdb3cql/pdb
PDB entry 3cql coloured by chain, front view.
PDB entry 3cql coloured by chain, side view.
PDB entry 3cql coloured by chain, top view.
Source organism: Carica papaya
Primary publication:
X-ray structure of papaya chitinase reveals the substrate binding mode of glycosyl hydrolase family 19 chitinases.
Huet J, Rucktooa P, Clantin B, Azarkan M, Looze Y, Villeret V, Wintjens R
Biochemistry 47 8283-91 (2008)
PMID: 18636748

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endochitinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 26.57 KDa
Source organism: Carica papaya
UniProt:
  • Canonical: P85084 (Residues: 1-243; Coverage: 100%)
Sequence domains: Chitinase class I
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NDG 2 x NDG
Ligand NAG 4 x NAG
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 69Å b: 44.49Å c: 76.81Å
α: 90° β: 94.97° γ: 90°
R-values:
R R work R free
0.162 0.161 0.187

Quick links

Citations

5 review citations

Biodegradation, biodistribution and toxicity of chitosan.
Kean et al. (2010)
4 more

8 mentions without citation

Chitinase: diversity, limitations, and trends in engineering for suitable applications.
Oyeleye et al. (2018)
7 more