› 3cro

X-ray diffraction
2.5Å resolution

THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb3cro/pdb
PDB entry 3cro coloured by chain, front view.
PDB entry 3cro coloured by chain, side view.
PDB entry 3cro coloured by chain, top view.
Source organism: Phage 434
Primary publication:
The phage 434 Cro/OR1 complex at 2.5 A resolution.
Mondragón A, Harrison SC
J Mol Biol 219 321-34 (1991)
PMID: 2038059

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116151 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Regulatory protein cro Chains: L, R
Molecule details ›
Chains: L, R
Length: 71 amino acids
Theoretical weight: 8.08 KDa
Source organism: Phage 434
UniProt:
  • Canonical: P03036 (Residues: 1-71; Coverage: 100%)
Gene name: CRO
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*AP*GP*TP*AP*CP*AP*AP*AP*CP*TP*TP*TP*CP*TP*TP*G P*TP*AP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Phage 434
Expression system: Not provided
DNA (5'-D(*TP*AP*TP*AP*CP*AP*AP*GP*AP*AP*AP*GP*TP*TP*TP*GP*T P*AP*CP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Phage 434
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-13
Spacegroup: P21
Unit cell:
a: 49.2Å b: 47.6Å c: 61.7Å
α: 90° β: 109.5° γ: 90°
R-values:
R R work R free
0.22 not available not available
Expression system: Not provided

Quick links

Citations

3 review citations

A structural taxonomy of DNA-binding domains.
Harrison SC. (1991)
2 more

26 mentions without citation

Bullied no more: when and how DNA shoves proteins around.
Fogg et al. (2012)
25 more