› 3cui

X-ray diffraction
1.5Å resolution

Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with sulfur substituted beta-1,4 xylotetraose

Released:
DOI: 10.2210/pdb3cui/pdb
PDB entry 3cui coloured by chain, front view.
PDB entry 3cui coloured by chain, side view.
PDB entry 3cui coloured by chain, top view.
Source organism: Cellulomonas fimi
Entry authors: Kuntz DA, Saul M, Rose DR

Function and Biology Details

Reactions catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Exoglucanase/xylanase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.27 KDa
Source organism: Cellulomonas fimi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07986 (Residues: 42-356; Coverage: 71%)
Gene names: cex, xynB
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: TWY, XYP
Ligand TWY 3 x TWY
Ligand XYP 1 x XYP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41212
Unit cell:
a: 85.7Å b: 85.7Å c: 78.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.133 0.167
Expression system: Escherichia coli

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