› 3cvl

X-ray diffraction
2.15Å resolution

Structure of Peroxisomal Targeting Signal 1 (PTS1) binding domain of Trypanosoma brucei Peroxin 5 (TbPEX5)complexed to T. brucei Phosphofructokinase (PFK) PTS1 peptide

Released:
DOI: 10.2210/pdb3cvl/pdb
PDB entry 3cvl coloured by chain, front view.
PDB entry 3cvl coloured by chain, side view.
PDB entry 3cvl coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the recognition of peroxisomal targeting signal 1 by Trypanosoma brucei peroxin 5.
Sampathkumar P, Roach C, Michels PA, Hol WG
J Mol Biol 381 867-80 (2008)
PMID: 18598704

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127541 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peroxisome targeting signal 1 receptor PEX5 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.55 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U7C3 (Residues: 332-655; Coverage: 50%)
Sequence domains: TPR repeat
Structure domains: Tetratricopeptide repeat domain
ATP-dependent 6-phosphofructokinase Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 841 Da
Source organism: Trypanosoma brucei brucei
Expression system: Not provided
UniProt:
  • Canonical: O15648 (Residues: 481-487; Coverage: 1%)
Gene name: pfk

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 44.39Å b: 66.36Å c: 49.68Å
α: 90° β: 105.13° γ: 90°
R-values:
R R work R free
0.215 0.212 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Structural and functional discussion of the tetra-trico-peptide repeat, a protein interaction module.
Zeytuni et al. (2012)
10 more

3 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
2 more